Interaction of DNA Minor Groove Binder Hoechst 33258 with Bovine Serum Albumin
نویسندگان
چکیده
منابع مشابه
Interaction of DNA minor groove binder Hoechst 33258 with bovine serum albumin.
Hoechst 33258 belongs to bisbenzimidazole class of molecules having anticancer properties for their ability to inhibit topoisomerase and many other cellular processes. The aim of the present study is to understand the nature of Hoechst 33258-bovine serum albumin (BSA) binding interactions by using absorption, fluorescence and circular dichrorism (CD) measurements under simulative physiological ...
متن کاملInteraction of Phthalocyanine with Egg albumin and Bovine serum albumin
The interaction of bovine serum albumin ( BSA) and egg albumin with water solublephthalocyanine, cobalt (ΙΙ) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( CoTSPc) , has been studiedby the UV- Vis method at pH 7.0 and five different temperatures 20, 25, 30, 35 and 40°C. Theformation constants have been elucidated by using spectrophotometric titration and computerSQUAD program data refinement. Th...
متن کاملInteraction of Hoechst 33258 and ethidium with histone1-DNA condensates.
We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of compact-globular and hollow-toroidal particles. In order to explore the functio...
متن کاملHoechst 33258, distamycin, and netropsin are among a number of compounds that bind in the minor groove of DNA
number of compounds that bind in the minor groove of DNA and exhibit various degree of antitumour activity. Several high resolution crystal and NMR structures have been determined for these ligands bound to short oligonucleotides and provide valuable insights into the details of their interaction. The Hoechst 33258 is bound in the minor groove, with the planar benzimidazole groups oriented para...
متن کاملQuantitation of DNA using Hoechst 33258
An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 2009
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.57.481